Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Inositol

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Description

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without InositolInositol is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of inositol or to replace this vitamin. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room temperature

CAS 69-52-3

2 gram powdered medium in 1 litre distilled water

3g) in one litre is 10X

VL6 is composed of a Vegetable peptone providing high molecular weight peptides and proteins as a nitrogen source

Component g/l Soya Peptone 16 Yeast extract 10 NaCl 5 Final pH 7

Tryptone 16 Yeast extract 10 NaCl 5 Final pH 7

2 at 25°C Suspend 23 gram powdered medium in 1 litre distilled water

TRIS Buffer 1

Enterobacter

Pre-Sporulation Medium is used for strains that do not sporulate well when grown on sporulation medium directly

4 which we determine during production

at this strength the ph is around 6