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Description

Yeast Nitrogen BaseYeast Nitrogen Base is based upon the formulation of Yeast Agar except that Ammonium Sulfate is the sole nitrogen source and that addition of carbon source is required. The medium may be used to test carbon assimilation in the presence of a carbon source like glucose, galactose or raffinose. Suspend 6. 9 gram powdered medium in 1 litre distilled water Store dry at room temperature

Trace elements and Vitamins without Agar and Amino Acids

1 g/L SDS 69

7 NH4Cl 26

In LBM media Mg2 is added to enhance adsorption of phage lambda to the cells

1 gram powdered medium in 1 litre distilled water Store dry at room temperature

Potassium phosphate is present in Terrific broth buffered to balance the pH and subsequently prevent cell death due to a drop in pH

2 at 25°C Suspend 43

2 at 25°C Suspend 47

0 Beef Extract 5

MES 1 M 195

Chemical formula C5H11NO2S Solubility (20° C) 48 g/l Mol

50 X sterile stock solution